Figure 2.

Schematic description of the hypothesized and miR-10a in human tissues. (A) Schematic description of the hypothesized duplex formed by interaction between the BCL6 3′-UTR (top) binding site and miR-10a (bottom). The predicted free energy value of the hybrid is indicated. The seed recognition site is denoted, and all nucleotides in this region are highly conserved across species, including Homo sapiens, chimp, rhesus, and mouse. (B) Quantitative RT-PCR analysis of the expression levels of miR-10a, the relative expression levels were analyzed using ΔCt values (ΔCt = Ct_miR-10a − Ct_U6). The U6 gene served as the endogenous control in diffuse large B-cell lymphoma(DLBCL) and reactive lymph node hyperplasia (RLH) tissues. Data (mean ± SEM) are representative of 3 technique replicates. ** P < 0.01