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. 2004 Sep 28;2(10):e301. doi: 10.1371/journal.pbio.0020301

Figure 1. P-Sig and Q-Sig Defined by Gene Expression Levels in HSCs in Different Stages of Cell Cycle.

Figure 1

(A) Graphic depicting the changes in bone marrow cellularity and number of HSCs in cell cycle following 5FU treatment (adapted from Harrison and Lerner 1991; Randall and Weissman 1997).

(B) Schematic of 5FU-HSC time course analysis. The genes that change over the time course can be split into two groups based on the day of maximum expression (TOM).

(C) Schematic of pair-wise comparison between quiescent adult HSCs and FL-HSCs, showing groups of genes either up-regulated in the quiescent adult cells or up-regulated in the cycling FL-HSCs.

(D) Genes that were both up-regulated in FL-HSCs and were in the proliferation group composed the P-sig. The P-sig shows 94% overlap with the group of genes that were up-regulated in FL-HSCs and changed over the time course.

(E) Genes that were both in the quiescence group and up-regulated in adult HSCs were termed the Q-sig. The Q-sig overlaps 96% with the set of genes that were up-regulated in adult HSCs and changed over the time course.

(F) Overlap of the ST-HSC signature with P-sig revealed 73% in common, defining the common P-sig.

(G) Overlap of the LT-HSC signature with Q-sig revealed 58% in common and defined the common Q-sig.

This figure is interactive online, and provides contextual access to Tables S1–S11. Use your mouse to highight animated areas of the graphic. Click on these areas to link to related files.