Fig. 1.

Expression of Cl⁻ and HCO₃⁻ transporters. qPCR was performed on extracts of unsorted cells and interstitial cells of Cajal (ICC) (B) and smooth muscle cells (SMC) (A) purified by fluorescence-activated cell sorting. Primers used are provided in Table 1. Inset: portion of the main graph outlined to show an expanded scale for several transcripts. Slc12a2 (Na⁺K⁺Cl⁻ cotransporter, NKCC1) transcripts were highly enriched in ICC. The data also showed somewhat enhanced expression of Slc4a2 and Slc4a4 in ICC. Relative expressions of all transcripts were normalized to Gapdh.