Fig. 5.

Effects of bumetanide on spontaneous transient depolarizations (STDs) in ICC. Cells were studied under current-clamp conditions (I = 0) using perforated whole-cell patch-recording conditions. CaPSS was used as the external solution (Solution I), and the K⁺-rich solution (Solution II) was used as the pipette solution. A shows STDs recorded under control conditions and the effects of bumetanide (40 μM). Bumetanide progressively decreased the maximal level of depolarization that occurred during STDs. Ba and Bb show portions of the extended trace in A at expanded time scales. These excerpts are taken at the points denoted by a and b in A.