Table 1.
Primers used for qPCR
| Gene | Primer Sequence | Accession No. |
|---|---|---|
| mGapdh-F | TGAACGGATTTGGCCGTATTG | NM_001289726 |
| mGapdh-R | GATGGGCTTCCCGTTGATGA | |
| mSlc12a1-F all isoforms | CGGCAAGCTGAACATTACCA | NM_183354 |
| mSlc12a1-R all isoforms | TTAAACTGAGCGCTGGCTTC | |
| mSlc12a1-F isoform A | AGTCATGCTCTTCATTCGCC | NM_183354 |
| mSlc12a1-R isoform A | CTCCACCTCCACGAACAAACC | |
| mSlc12a1-F isoform B | GGGAATTGGTCTCGGTGTGA | U20974 |
| mSlc12a1-R isoform B | GGCTCCACCTCCTCTGACAT | |
| mSlc12a1-F isoform F | ATCATTGGCCTGAGCGTAGT | NM_001079690 |
| mSlc12a1-R isoform F | AAGCCTATTGACCCACCGAA | |
| mSlc12a2-F | CTTGCGAGAAGGTGCACAATA | NM_009194 |
| mSlc12a2-R | TCAGGCGGATAACCACAACT | |
| mSlc12a3-F | CCCTTGTGGACTTTGTGAGC | NM_001205311 |
| mSlc12a3-R | TTGTTCAGCCACTTGGTGTG | |
| mSlc4a1-F | ACCTAACCATCCCTGTGACC | NM_011403 |
| mSlc4a1-R | CCACATAGACCTGACCGGAG | |
| mSlc4a2-F | CTGGAGGTGGATAGAGAGCG | NM_009207 |
| mSlc4a2-R | CTCCGCATTCTCAGGGATCT | |
| mSlc4a3-F | CTTTGAGTTTCACCGGCACA | NM_009208 |
| mSlc4a3-R | GATAGGGGGTGTCCCTTCTG | |
| mSlc4a4-F | CAACGACGATTCTGACTGCC | NM_053424 |
| mSlc4a4-R | TGTGTGGCGTTCAAGGAATG | |
| mSlc4a5-F | ACACAGACCAGCGGAAAAAC | NM_001166067 |
| mSlc4a5-R | GCAGACTGGACAAGACGAAC | |
| mSlc4a7-F | CAGGAGAGTGGGATCCTTCC | NM_001033270 |
| mSlc4a7-R | CAAAAAGCCGTCCAGTCCTC |
F, forward; R, reverse.