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The composition of solutions
| Solutions (mM) | I | II | III | IV | V | VI |
|---|---|---|---|---|---|---|
| KCl | 5 | 135 | 5 | 0 | 0 | 0 |
| NaCl | 135 | 0 | 0 | 0 | 0 | 0 |
| Na isethionate | 0 | 0 | 135 | 0 | 0 | 0 |
| CsCl | 0 | 0 | 0 | 135 | 0 | 0 |
| NMDGCl | 0 | 0 | 0 | 0 | 147.7 | 150 |
| CaCl2 | 2 | 0 | 2 | 0 | 4.14 | 2 |
| MgCl2 | 1.2 | 0 | 1.2 | 0 | 0 | 1 |
| MgATP | 0 | 3 | 0 | 3 | 0 | 0 |
| NaGTP | 0 | 0.1 | 0 | 0.1 | 0 | 0 |
| CPD | 0 | 2.5 | 0 | 2.5 | 0 | 0 |
| Glucose | 10 | 0 | 10 | 0 | 0 | 0 |
| EGTA | 0 | 0.1 | 0 | 0.1 | 10 | 0 |
| HEPES | 10 | 10 | 10 | 10 | 10 | 10 |
| pH | 7.4 | 7.2 | 7.4 | 7.2 | 7.2 | 7.4 |
All solutions were adjusted for pH with Tris. CPD, creatinine phosphate disodium.
