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Hydrogen peroxide (H2O2) induces 2-AG levels in THP-1 cells. THP-1 cell lysates (A) or intact living THP-1 cells (B) were treated with H2O2 (1 mM, 60 min), followed by 2-AG quantitation. Cell lysate was also treated with Ca2+ to stimulate 2-AG synthesis (as a positive control). The vehicle (Veh) for H2O2 was water. Values are means ± SD (n = 2–3). *P < 0.05 (relative to vehicle).
