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. 2016 Oct 26;311(6):C931–C941. doi: 10.1152/ajpcell.00180.2016

Fig. 5.

Fig. 5.

Preexposure to acidic water increases the number of Sodium Green-positive cells that are not HRCs. A–C: at 4 days postfertilization, accumulation of Sodium Green was predominantly confined to HRCs (labeled by concanavalin A) in the skin of larval zebrafish following exposure to control water (pH 7.6). D–F: after preexposure to acidic water (pH 4.0), accumulation of Sodium Green was observed in epithelial cells that were not HRCs. Scale bar = 25 μm. Quantitative analysis revealed that the total number of Sodium Green-positive cells (G) and the numbers of Sodium Green-positive non-HRCs (H) were significantly increased after acid exposure. I: however, acid exposure did not affect the number of Sodium Green-positive HRCs. Values are means ± SE. *Statistical difference between groups (Student's t-test, N = 6), P < 0.05.