Fig. 6.

The increased number of Sodium Green-positive cells by acute acid exposure is not observed in fish experiencing NCC knockdown. A: compared with shams, the number of NCC-positive cells was significantly lowered than that in NCC morphants (NCC MO) at 4 days postfertilization. The inset images showed the immunohistochemistry of NCC in sham and NCC MO. B–D: following exposure to control water (pH 7.6), Sodium Green was predominantly accumulated in HRCs (labeled by concanavalin A) on the skin of NCC MO (depicted by arrows). E–G: similarly, Sodium Green accumulation was confined to HRCs of NCC MO following preexposure to acidic water (pH 4.0). Scale bar = 25 μm.