Fig. 2.
pHe-dependence of membrane conductance (Gm) in H2O-injected and mSlc4a11-expressing oocytes. A–C: representative sets of current-voltage (I–V) relationships for an oocyte that had been injected with H2O (A), mSlc4a11 cRNA (B), or cRNA encoding an “R99H” mutant of mSlc4a11 (C). A representative current ladder obtained for the Slc4a11-expressing oocyte bathed for 20 min in pHe = 8.5 solution is shown in the inset in B. D: average membrane conductance calculated for six of each cell type from data such as that in A, B, and C. Letters above each bar denote statistically indistinguishable groups. The inset in D shows one of three Western blots of biotinylated oocyte protein, probed for mSlc4a11 immunoreactivity.