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Composition (in mM) of solutions used in electrophysiological assays
| Solution |
|||||||||
|---|---|---|---|---|---|---|---|---|---|
| Na+ | NMDG+ | K+ | Ca2+ | Mg2+ | Cl− | Gluconate− | Buffer | pH | |
| pHe = 6.5 | 96 | 2 | 1.8 | 1 | 103.1 | 5 (Bis Tris) | 6.5 | ||
| pHe = 7.5 | 96 | 2 | 1.8 | 1 | 103.1 | 5 (HEPES) | 7.5 | ||
| pHe = 8.5 | 96 | 2 | 1.8 | 1 | 103.1 | 5 (Bicine) | 8.5 | ||
| Na-free | 96 | 2 | 1.8 | 1 | 103.1 | 5 (Bicine) | 8.5 | ||
| Cl-free | 96 | 2 | 1.8 | 1 | 103.1 | 5 (Bicine) | 8.5 | ||
