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. 2016 Sep 30;311(6):H1392–H1408. doi: 10.1152/ajpheart.00463.2016

Fig. 4.

Fig. 4.

Effects of the SR-BIΔCT (ΔCT) mutation on plasma total (TC) and unesterified (UC) cholesterol levels and the UC-to-TC ratio (UC:TC; A) and on plasma lipoprotein size distribution profiles (cholesterol and apolipoproteins; B). Plasma samples were harvested from male (M) and female (F) WT and SR-BIΔCT (ΔCT) 6- to 10-wk-old mice. A: plasma total (a) and unesterified (b) cholesterol levels and UC:TC (c) were determined in individual samples by enzymatic assay; mean (±SE) values from the indicated numbers of animals (n) are shown. *Significantly different (P < 0.0001) between ΔCT mice and the corresponding WT control mice of the same sex. B: pooled plasma samples from 3 males of each genotype were size-fractionated by FPLC, and the total cholesterol content of each fraction was determined by an enzymatic assay (top). The chromatograms are representative of multiple individually determined profiles. Approximate elution positions of native VLDL, IDL/LDL, and HDL particles are indicated by brackets and were determined as previously described (50). The FPLC fractions were analyzed by immunoblotting (bottom) to determine the distribution of the apolipoproteins apoA-I and apoE.