Fig. 6.

Effects of the SR-BIΔCT (ΔCT) mutation in apoE KO mice on plasma total (TC) and unesterified (UC) cholesterol levels and UC:TC (A) and on the plasma lipoprotein cholesterol size distribution profile (B). A: plasma samples were harvested from apoE KO and SR-BIΔCT/apoE KO 6- to 8-wk-old mice. No significant differences were observed between males and females. Total plasma and unesterified cholesterol levels were determined in individual samples by enzymatic assay; mean (±SE error) values from the indicated numbers of animals (n) are shown for each genotype. *P < 0.0001 for SR-BIΔCT/apoE KO compared with apoE KO mice. B: pooled plasma samples from 3 male mice were size-fractionated by FPLC, and the total cholesterol content of each fraction was determined by an enzymatic assay. The shapes of the chromatograms are representative of multiple individually determined profiles. Approximate elution positions of native VLDL, IDL/LDL, and HDL particles are indicated by brackets and were determined as previously described (50).