Fig. 6.

H₂S recovery in PBS and 10% albumin solution after 1 h of incubation with 200 μM H₂S (data obtained at 10 min are given for comparison and are the same as in Fig. 5). Data presented similarly to Fig. 5. A: in PBS, recovery of free H₂S was 97% and 83% of original concentration after 10 min and 1 h of incubation, respectively. TCEP (5 mM) and HCl (0.3 M) injection did not produce any significant levels of H₂S. In albumin, 94% of initial concentration of free H₂S was not recovered after 1 h of incubation. H₂S produced by TCEP or HCl corresponded to a tiny proportion of H₂S that was not recovered. *P < 0.05, significant difference between 10-min and 1-h incubation. B: various pools of H₂S recovered from PBS or albumin solution after 10-min and 1-h incubation. This representation illustrates that the major part of H₂S “disappearing” from H₂S mixed with albumin was not recovered after TCEP or HCl.