Figure 5.

Effects of BMSCs on bortezomib (BTZ)-mediated MM cell apoptosis in vitro. RPMI 8266 cells were cultured in medium containing 20 nmol/l BTZ for 24 h and the apoptosis of MM cells was determined by FCM analysis, as shown in 5 E. The rate of apoptosis of MM cells cultured in the absence of BMSCs was approximately 76% (D), but the ratio percentage of apoptosis decreased significantly when MM cells were co-cultured with BMSCs (p < 0.05) (B and C), and increased when treated with 50 mM/l heptanol compared with the controls (4 and 6). A – Negative control