Table 4.

Top 3 DAVID GO functional annotation charts comparing each liver condition (Steatosis vs. Normal, NASH vs. Steatosis, NASH vs. Normal) constructed using all upregulated and downregulated mRNAs in IAT

	Upregulated GO Categories		Downregulated GO Categories
GO Category	Top Function	mRNAs Involved	Top Function	mRNAs Involved
Steatosis vs. Normal
Biological process	none identified	No upregulated networks identified	translational elongation	EEF1B2, RPL28, RPL31, RPL34, RPL38, RPL6, RPL8, RPL9, RPS14, RPS24, RPLPO, RPL32
Cellular component	none identified	No upregulated networks identified	cytosolic ribosome	RPL28, RPL31, RPL34, RPL38, RPL6, RPL8, RPS14, RPS24, RPLPO, RPL32
Molecular function	none identified	No upregulated networks identified	structural constituent of ribosome	RPL28, RPL31, RPL34, RPL38, RPL6, RPL8, RPS14, RPS24, RPLPO, RPL32
NASH vs. Steatosis
Biological process	immune response	OAS2, CD209, CD79B, CD83, FAIM3, FCER1G, GPR183, GPSM3, GCH1, ARHGDIB, SP100, ST6GAL1, WAS, ACP5, APOBEC3F, CNPY3, CCL19, CCL21, CCL5, CXCR4, CITA, C1QA, CIQB, CIQC, CFP, GZMA, IGJ, ICOSLG, INPP5D, ITGAL, IRF8, IL18BP, LILRB1, LILRB4, LST1, LAT2, LY86, LCP1, LCP2, LTB, HLA-B, HLA-F, HLA-H, HLA-J, HLA-DMA, HLA-DOA, HLA-DRB1, NCF1, NCF4, SEMA4D, TLR1, TFEB, TAP1, TAP2, TNFRSF14, UNC13D, ETS1	vasculature development	APOL D1, FGF2, ENPEP, HEY1, HAND2, MMP2, PRRX2, PLAT, RHOB, TCF21
Cellular component	plasma membrane	ABCB7, ADAP1, BMF, CD163L1, CD209, CD33, CD37, CD52, CD53, CD79B, CD83, CDC42SE1, CDC42SE2, EVL, FAIM3, FCER1G, GPR160, GPR183, NCKAP1L, NFAM1, RAB7L1, RASGRP2, RASA4, ARHGAP4, ARHGEF1, SHKBP1, ADCY7, ADRBK1, AIF1, CSK, CASP8, CXCR4, CSF2RB, CYTH1, DGKD, DGKZ, DEF6, DOK3, EVI2B, FERMT3, FLOT2, FNBP1, GZMA, GNG7, ITGAL, ITGAX, ITGB2, ICAM3, IL10RA, KLB1, LILRB1, LILRB4, LAT2, LY86, LCP1, LAPTM5, HLA-B, HLA-F, HLA-H, HLA-J, HLA-DMA, HLA-DOA, HLA-DRB1, MYO7A, NKG7, NCF1, NCF4, OPN3, PREX1, PIK3AP1, PLEK, PLEKHA2, PLEKHO1, PARP14, PAQR8, PPP1R16B, PTPRCAP, PTPRE, PTPREJ, P2RY13, RGS19, SELL, SEMA4D, SPINT2, SIGLEC1, GFRA2, SLC2A1, SLC2A3, SYNGR2, SYT15, TLR1, TAP1, TAP2, TNFRSF14, TNFRSF 25, VAMP1	extracellular region	ADAMTS1, ADAMTS16, SPARCL1, AZGP1, ANGPTL7, APOLD1, CPE, CXORF36, CRISPLD2, DEFB132, FGF2, FBLN1, GSN, GPC6, SSC5D, IGFBP5, LUM, MMP2, MXRA5, NGF, NTNG1, OLFML3, PLAT, SFRP1, SFRP2, SERPINI1
Molecular function	GTPase regulator activity	ACAP1, ADAP1, CDC42SE1, FGD2, FGD3, GIT2, GPSM3, GMIP, IQGAP2, RASGRP2, RASA4, RIN3, ARHGDIB, ARHGAP15, ARHGAP25, ARHGAP27, ARHGAP30, ARHGAP4, ARHGAP9, ARHGEF1, ARHGEF18, SH3BP1, WAS, CYTH1, CYTH4, DOCK10, DOCK2, DOCK8, MYO9B, PREX1, RABEP2, SIPA1	glutathione transferase activity	GSTM1, GSTM2, GSTM3, GSTM5
NASH vs. Normal
Biological process	dephosphorylation	ACP5, DUSP5, PTPN1, PTPRE, SYNJ1	negative regulation of cell proliferation	ADAMTS1, CD164, KLF4, SMARCA2, AZGP1, ANG, ASPH, CDKN1C, CDKN2C, FGF2, GPNMB, IGFBP3, ILK, NME1, NUPR1, NPM1, PMP22, PPARG, PTPRF, TENC1
Cellular component	intrinsic and integral to membrane	ATP13A2, CD163L1, CD209, CD83, GPR137, GM2A, LFNG, TAPBP, ACP5, APLNR, BTN3A1, BT3A2, C19ORF28, CYBASC3, CYB561D1, FAM118A, FLOT2, FFAR2, FZD8, BAMBI, ITPR1, ITM2C, ICAM2, LPCAT1, MP EG1, NKG7, NCF4, NUP50, PCNXL3, PPAPDC1B, PTDSS1, PLA2G4C, PCSK7, PPP1R16B, PTPRE, RNF122, SLC12A6, SLC2A3, SLC37A1, VAMP1	cytosol	AGPAT2, PFKFB3, ARF1, BCAP31, BAD, PARK7, TPI1, ACACB, ACSL1, ADH1A, CTNNB1, CHMP2A, CYB5R3, EIF3B, EIF3C, EIF4H, FASN, GSN, GLUL, GPT, GPX4, GYG2, GUK1, HSPD1, HSP90AA1, HK2, LDHA, KARS, MOCS2, NME1, NFKBIA, NPM1, PRDX6, PPARG, RAC1, RPL31, RPL9, RPS20, RPS24, RPLPO, SQSTM1, SDPR, YWHAE, BANF1, SNRPD2, SORBS1, SDCBP, VCL
Molecular function	lipid binding	DGKZ, FFAR2, HIP1R, ITPR1, NCF4, PLA2G4C, PTGDS, PRKD2	glycosaminoglycan, polysaccharide, and pattern binding	ADAMTS1, ANG, CYR61, DCN, FGF2, GPNMB, LTBP4, PRELP, VEGFA

Gene Ontology (GO) biological process, cellular component, molecular function, and KEGG themes enriched in intra-abdominal adipose tissue (IAT) and were annotated by GO themes using FunNet. Underlined transcripts were downregulated.