Fig. 1.
Study designs and treatment protocols. Two-day-old Sprague-Dawley rats received daily bleomycin (0.5 mg/kg) or 0.9% saline by intraperitoneal injection for 14 days. In addition, 2-day-old rat pups also received BQ123 and BQ610 (0.5 and 1 mg/kg) (selective ETA receptor blocker), BQ788 (0.5 and 1 mg/kg) (selective ETB receptor blocker), bosentan (10 and 100 mg/kg) (nonselective endothelin receptor blocker), and fasudil (15 mg/kg) (Rho kinase inhibitor) alone and in combination with bleomycin. Animals were killed at 14 days and lungs and hearts were harvested for histology, morphometric analysis, vessel density, medial wall thickness, and assessment of RVH.