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Rac1 inhibition alters lung vasculogenesis ex vivo. A and B: LacZ staining on Flk1-LacZ reporter explants cultured in absence (A) or presence (B) of Rac1 inhibitor. C and D: immunofluorescence for CD31 (red) of untreated (C) and Rac1 inhibitor treated (D) lung explants; blue is DAPI. E: quantification of Vegfa by qRT-PCR in control and treated lung explants. Data are represented as means ± SE of at least 7 independent experiments; *P = 0.0007. Scale bar is 50 μm.
