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Wif1 inactivation is not sufficient for rescuing lung branching due to Rac1 inhibition. A–D: E12.5 lung explants from Wif1−/− mice cultured in absence (A and B) or presence (C and D) of NSC23766 at t = 0 (A and C) and t = 48 h (B and D). E: quantification of the fold change in number of buds between 48 h and 0 h of control and NSC23766-treated lungs. Data are represented as means ± SE of 8 independent experiments. *P ≤ 0.05.
