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. 2016 Dec 20;2016:6253280. doi: 10.1155/2016/6253280

Figure 2.

Figure 2

Emodin inhibited EMT in A2780 and SK-OV-3 cells through targeting Slug. (a) Different concentration of emodin exposure for 48 hours upregulated the protein expression of E-cadherin and Claudin and downregulated protein expression of N-cadherin, Vimentin, and Slug in A2780 and SK-OV-3 cells. (b) Quantitative analyses of E-cadherin, N-cadherin, and Slug in SK-OV-3 cells shown in (a). ∗ P < 0.05, ∗∗ P < 0.005, and ∗∗∗ P < 0.001. (c) A2780 and SK-OV-3 cells were transfected with siRNA of Slug and cultured for an additional 48 h treated with emodin 20 μM or without emodin. Western blot analysis results of Slug, E-cadherin, and N-cadherin protein levels were shown. (d) Quantitative analyses of markers in SK-OV-3 cells shown in (c). ∗ P < 0.05, ∗∗ P < 0.005, and ∗∗∗ P < 0.001. # P < 0.05. (e) Representative transwell migration and invasion assay of A2780 and SK-OV-3 cells after transfection with siRNA of Slug with or without emodin treatment. (f) Quantification of migration and invasion capacity of SK-OV-3 cells in (e). ∗∗ P < 0.005 and ∗∗∗ P < 0.001. # P < 0.05 and ## P < 0.005. N.C. represents transfection of siRNA-control.