Fig. 3.

Dissecting the role of Rac1 in the regulation of Src mediated morphological changes. Effect of Rac1 inhibition on Src mediated (A) protrusive activity and (B) cell spreading. HeLa cells transfected with: RapR-Src-as2-cerulean-myc, iPEP-mCherry-FRB, and Stargazin-mVenus were imaged live. Stargazin-mVenus images were used to assess cell area and protrusive activity. (A and B) Cells were treated with rapamycin (500 nM) (green line), and 15 min later cells were treated with either DMSO (solvent), 1NA-PP1 (250 nM), or 1NA-PP1+Rac1-I (1NA-PP1+CAS 1177865–17-6, 250 nM + 100 μM) as indicated by red triangle or line. All error bars/shading represent 90% confidence intervals. (C) Schematic depicting different roles for Rac1 signaling at different stages upon transient activation of Src.