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. 2016 Dec 12;113(52):14976–14981. doi: 10.1073/pnas.1609675114

Fig. 5.

Fig. 5.

Dissecting the role of PI3K in the regulation of Src-mediated morphological changes. (A and B) Effect of PI3K inhibitor on cell spreading (A) and protrusive activity (B). HeLa cells transfected with RapR-Src-as2-cerulean-myc, iPEP-mCherry-FRB, and Stargazin-mVenus were imaged live before and after the addition of rapamycin (green line). Stargazin-mVenus images were used to evaluate changes in cell area and protrusive activity. (A) Cells were treated with DMSO (solvent) or 1NA-PP1+Wortmannin (250 nM, 100 nM) at the indicated time points (red triangles) after addition of rapamycin. (B) Cells were treated with the DMSO (solvent), 1NA-PP1 (250 nM), or 1NA-PP1+LY294002 (250 nM + 50 μM) (red triangle) 15 min after rapamycin treatment. (C) Schematic depicting different roles for PI3K signaling at different stages upon transient activation of Src.