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. 2016 Dec 13;113(52):14970–14975. doi: 10.1073/pnas.1609375114

Fig. S5.

Fig. S5.

Purity and storage stability of produced Fabs and size exclusion chromatography (SEC) analysis of Fab 3A2. (A) Nonreducing SDS/PAGE of purified long CDR-H3 Fabs. Typical yields of Fabs were 0.5–2 mg/L in E. coli after purification. Trace amounts (typically <2%) of unassembled VHs are presented at ∼27 kDa. (B) SEC of purified Fab 3A2 using an analytical Superdex 75 10/300 GL column (GE Health). Fab 3A2 was eluted as a monomer, without any detectable level of aggregates. (C) Nonreducing SDS/PAGE of purified Fabs after storage for 1 mo at 4 °C.