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Stability of purified Fabs in the presence of MMP-14 at an enzyme/Fab ratio of 1:5 or 1:10. Mixtures at pH 7.5 were incubated at 37 °C for 1 h or overnight (O/N; 16 h). Except for the Fab 3A2 overnight sample at the 1:5 ratio, none of the other tested conditions resulted in significant cleavage of Fabs by MMP-14. M, molecular weight standard.
