Fig. 1.

Genetic mapping of Pms1. (A) Fertility phenotypes of 58S and NIL(MH) under long- and short-day conditions. Whole plants are shown (Left), panicles (Center), and pollen (Right). (B and C) Spikelet fertility of plants (B) and their frequency distribution (C) in the F₂ population from a cross between 58S and NIL(MH) under long-day conditions. The plants were genotyped using marker Fssr. (D) Mapping of Pms1 to a 4.0-kb region on chromosome 7 according to the MH63 genome sequence. The black blocks in the middle represent exons, and the lines between them represent introns, arrows indicate the directions of genes. Sequence polymorphisms between two parents MH63 and 58S are shown below, where vertical lines represent the single nucleotide polymorphisms, and triangles for insertions. The gray block at the bottom indicates the fragment amplified from 58S for preparing the construct 58S-C.