Table S1.
Spikelet fertility of T1 families from two representative T0 transgenic plants under long-day conditions
| Construct | Genotype | Spikelet fertility, % | |
| Family 1 | Family 2 | ||
| Ubi:S | Positive | 0.39 ± 0.13 (24) | 0.19 ± 0.07 (29) |
| Negative | 73.34 ± 1.67 (16) | 68.46 ± 2.54 (9) | |
| P value | 0.0000 | 0.0000 | |
| 35S:S | Positive | 14.72 ± 2.90 (27) | 35.51 ± 4.89 (15) |
| Negative | 66.82 ± 3.58 (8) | 66.18 ± 1.14 (3) | |
| P value | 0.0000 | 0.0146 | |
| 35S:S-CS | Positive | 68.15 ± 1.33 (18) | 71.52 ± 1.28 (20) |
| Negative | 65.51 ± 1.83 (8) | 71.77 ± 4.14 (5) | |
| P value | 0.2706 | 0.9396 | |
| Ubi:S CS+P6 | Positive | 8.94 ± 0.76 (33) | 0.18 ± 0.06 (20) |
| Negative | 74.41 ± 3.44 (13) | 70.57 ± 1.83 (16) | |
| P value | 0.0000 | 0.0000 | |
| Ubi:S P6 | Positive | 73.72 ± 1.58 (22) | 69.51 ± 2.66 (26) |
| Negative | 77.70 ± 3.98 (7) | 73.15 ± 1.84 (4) | |
| P value | 0.2736 | 0.6024 | |
| Ubi:S P6+polyA | Positive | 72.93 ± 1.40 (20) | 80.94 ± 1.31 (17) |
| Negative | 73.25 ± 1.40 (9) | 82.58 ± 1.44 (12) | |
| P value | 0.8890 | 0.4128 | |
| 58S-C-dP6 | Positive | 0.34 ± 0.10 (21) | 21.14 ± 4.31 (33) |
| Negative | 62.99 ± 6.05 (8) | 73.44 ± 1.28 (12) | |
| P value | 0.0000 | 0.0000 | |
| 58S-ORF1+G | Positive | 1.35 ± 0.30 (33) | 21.30 ± 5.38 (29) |
| Negative | 64.65 ± 5.70 (9) | 67.92 ± 2.42 (12) | |
| P value | 0.0000 | 0.0000 | |
| 58S-ORF2+G | Positive | 1.34 ± 0.22 (33) | 32.93 ± 4.06 (30) |
| Negative | 80.43 ± 1.04 (16) | 79.74 ± 1.05 (6) | |
| P value | 0.0000 | 0.0000 | |
| 58S-ORF3+G | Positive | 3.36 ± 2.40 (28) | 1.50 ± 0.49 (27) |
| Negative | 79.90 ± 3.75 (7) | 79.90 ± 2.59 (8) | |
| P value | 0.0000 | 0.0000 | |
All data presented are mean ± SEM. Numbers in parentheses are numbers of plants analyzed. The P value is obtained from the t test of positive and negative plants. All constructs were transformed into NIL(MH).