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. 2016 Dec 13;113(52):15144–15149. doi: 10.1073/pnas.1619159114

Table S4.

Primers used in this study

Name Sequence (5′ to 3′) Remark
Markers for map-based cloning
 pj23-F AGAGAGAACTCTGCCAATATC SSR
 pj23-R AAAGTAACCCCTGCATAGACT
 P1-F TCCTGTGGTCCAATCAAACG CAPS, MunI
 P1-R CAGATCGTCTGATGATGTTGC
 P2-F GCAATGGCTGTTGTCCGAAT CAPS, Cfr10I
 P2-R CTATGACGTTTGCTTTGTGCC
 P3-F CTCCTTCGCAGACTCTACAC CAPS, Eco52I
 P3-R ATGTCAATCTCTGATATCGGC
 P4-F GCGAGCACATAAGTCCTCTA dCAPS, AluI
 P4-R ATAATTGTTTAAAGGTGTCAG
 P5-F AGGCGCAGTAAAAACACCTG SSR
 P5-R CTTGCGTGGTTTCAAGGG
 P6-F CATTAGGCGGAGATGGCAAT InDel
 P6-R ATAGCCAACACTCATCACTGTCG
 P7-F AAGGAGATGCAGAGAGGGTTTAC InDel
 P7-R AGGAAGGATAGGTGTTTGATGAAC
 P8-F TCAGATGACTTAGCAACACAAGC CAPS, DraI
 P8-R CTTGCTTATCCCAAATGTCGT
 P9-F AGGAAACGATAGGCGAACAAAC CAPS, EcoRV
 P9-R ACAAGGCTTCCAAACTTCCAA
 P10-F CACGACCTTACTTATGAGCATG SSR
 P10-R AAAAGAACACAGCAGGGGAG
 P11-F GTCCCTCATCTTTAGGCTGTG SSR
 P11-R GCCTAAAGAGGAGGGACGAA
 Fssr-F TTAGTGCGTGTGGCATAGATG SSR
 Fssr-R GGCCTTTTTGTGAATTGGG
Vector construction
 58S-C-F ATGAAGGACCGAGAAGAAGC 58S-C
 58S-C-R GTTCTCAGATGATATGGAACTGTG
 dsi-F TCGACTAGTTGGCAGGGTACCTGTACATGCCCAACAAGCTCT dsi
 dsi-R GGCGAGCTCGGATCCTGTTCGCATGGACAATTGGTG
 OX-1F TATGGTACCGACTACATGGGCACCCCTTGAA 35S:S, Ubi:S
 OX-1R TATGGATCCCGTGATTCAGCAGGTGGAGTTAA
 OX-2F TCAGAGCTCGCATCAGGAAAGAAGCTTCTACTA 35S:S-CS
 OX-2R TGACCTGCAGTACTTCAGTACCATCAGCGTGAC
 OX-3F TATGGTACCAATTGTCCATGCGAACAGAAAA Ubi:S P6
 OX-3R TATGGATCCCCGTAAACAATTATCGGTGATA
 ORF1G-R CATTAGGCGGAGATGGCAAT 58S-ORF1+G
 ORF1G-muF CAAAATCACAATAAAGATATATGGATCAAAACCTTAATAAT
 ORF1G-muR ATTATTAAGGTTTTGATCCATATATCTTTATTGTGATTTTG
 ORF1G-F AGGTGTCGGTTCTAATGGTAGAAC
 ORF2G-F CCATTTATAGGCTACTCCTTTCC 58S-ORF2+G
 ORF2G-muR AACTAGTATATAGTATATAGAGAAGCCCAAGTACCGAACTTTTTCTGTTCGCCAT
 ORF3G-R ATTGTTGCAGGTGAAGGTGAGC 58S-ORF3+G
 ORF3G-muF CCCATTGCCATCTCCGCCTAATGGAACGCCTTGCATGTTGG
 ORF3G-muR CCAACATGCAAGGCGTTCCATTAGGCGGAGATGGCAATGGG
 ORF3G-F CAATTTTGCCTGGTATCACCAA
Positive test
 PMCGF GGCTCACCAAACCTTAAACAA
 PMCGR CTGAGCTACACATGCTCAGGTT
 GUS1.6F CCAGGCAGTTTTAACGATCAGTTCGC
 GUS1.6R GAGTGAAGATCCCTTTCTTGTTACCG
 P6-F2 AATTGTCCATGCGAACAGAAAA
 P6-R2 CCGTAAACAATTATCGGTGATA
RACE
 RACE5-1 CTGATGACTGTGTTCCAGTATTTG 5′-RACE
 RACE5-2 ACGCAAGCTTGGCTCTTTGTT 5′-RACE
 RACE5-3 ACCTGGCATAGACCGATAGTTAC 5′ RLM-RACE
 RACE5-4 CTGATGACTGTGTTCCAGTATTTG 5′ RLM-RACE
 RACE3-1 CAATTTTGCCTGGTATCACCAA 3′-RACE
 RACE3-2 CAACATCATTAGGTTGCTGTGAT 3′-RACE
qPCR
 RT-F AAAGTTCGGTACTTGGGCTTCTCT
 RT-R ACTCCCATTCGATATTGTTGCAAGGGC
 UBQ-F AACCAGCTGAGGCCCAAGA
 UBQ-R ACGATTGATTTAACCAGTCCATGA

CAPS, cleaved amplified polymorphic sequences marker; dCAPS, derived cleaved amplified polymorphic sequences marker; InDel, insertion/deletion marker; SSR, simple sequence repeat marker.