Fig. 3.

TIFA is involved in signal 1 of NLRP3 inflammasome. (A) HUVECs were infected with Ad-null or Ad-TIFA. The protein level of phosphorylated p65 NF-κB, TIFA, caspase-1 p20, and IL-1β p17 was examined by Western blot. (B–F) HUVECs were transfected with TIFA siRNA (20 nM) or control siRNA (20 nM). (B) ECs were also infected with Ad-SREBP2. (C–F) ECs were stimulated with OS or TNF-α. The mRNA levels of pro–IL-1β mRNA and procaspase-1 were analyzed (B–D), along with the protein levels of TIFA, procaspase-1, caspase-1 p20, pro–IL-18 and IL-18 p18, or pro–IL-1β and IL-1β p17 (E and F). Data are mean ± SEM from three independent experiments (*P < 0.05).