Fig. S4.

Akt activates the TIFA-NLRP3 inflammasome axis in ECs. (A) HUVECs were transfected with control siRNA, TIFA siRNA, pCDNA3, or Akt E17K as indicated. The cells were then stimulated with or without LPS and ATP. Cells were lysed, and IL-1β cleavage was measured by Western blot. (B) HUVECs were transfected with Akt E17K and infected with Ad-null, Ad-TIFA WT, or Ad-TIFA T9A as indicated. Cells in the various experimental groups were lysed, and IL-1β contents were measured by ELISA (*P < 0.05).