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. 2016 Dec 12;113(52):14988–14993. doi: 10.1073/pnas.1616343114

Fig. 3.

Fig. 3.

TevCas9 generates deletions of precise lengths in HEK293 cells. Results from Illumina sequencing of PCR-amplified fragments for (A–C) NQO2, (D–F) TSC1.2125, (G–I) TSC1.5054, and (J–L) RARA target sites. (A, D, G, and J) Proportion of reads with length differences relative to the unmodified target, with blue triangles representing TevCas9, red open circles representing Cas9, and gray dots representing mock transfection. (B, D, F, and H) Proportion of TevCas9 reads with deletions mapped to the position in the target site. (C, F, I, and L) Proportion of Cas9 reads with deletions mapped to the position in the target site. Dotted vertical lines indicate I-TevI and Cas9 cleavage sites.