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Model of how TevCas9 biases DNA repair outcomes. TevCas9 or Cas9 recognizes and cleaves a target site. The noncompatible DNA ends and 33- to 36-bp deletion generated by TevCas9 prevents regeneration of the target site. Compatible DNA ends generated by Cas9 are repaired by NHEJ, regenerating the target site and inducing a cycle of cleavage and ligation. At a lower rate (dashed line), some Cas9 events undergo repair by the alt-NHEJ pathway, getting heterogeneous length indels.
