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Schematic overview of the glycolysis swapping approach. (A) Construction of SwYG that contains a single locus endogenous glycolysis platform for pathway swapping. (B) In silico design and in vivo assembly and integration of the glycolytic gene cluster on chromosome V, followed by the removal of the endogenous glycolysis on chromosome IX, leading to a strain with a redesigned glycolysis. M, selectable marker.
