Fig. 1.

Effects of β2ARKO on BM migration in response to chemokines. (A) Representative Hoechst staining (white) from a 4-h migration assay of WT BM, β2ARKO BM, β2ARKO BM+lenti-GFP, and β2ARKO BM+lenti-β2AR in response to CCL2 (100 ng/mL), CCL3 (100 ng/mL), or CXCL12 (10 ng/mL). A 1-h pretreatment with a CCR2 antagonist (10 nM) was used to inhibit CCR2-mediated migration. (B) Quantification of migration assay results. Values are expressed as fold over vehicle-stimulated migration. n = 4–8; one-way ANOVA, *P < 0.05 vs. WT BMT. (C) RT-qPCR was used to measure β2AR and CCR2 expression in WT and β2ARKO BM and β2ARKO BM that had been transduced with either a GFP or β2AR lentivirus. n = 4–8; one-way ANOVA, *P < 0.05 vs. WT BMT. Data are expressed as mean ± SEM. NS, nonstimulated; RQ, relative quantitation.