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. 2016 Dec 12;113(52):15126–15131. doi: 10.1073/pnas.1611023114

Fig. 6.

Fig. 6.

AP-1 activation is decreased in β2ARKO BM. (A) Representative EMSAs from nuclear WT and β2ARKO BM extracts incubated with an AP-1–specific probe. (B) A representative EMSA from nuclear WT and β2ARKO BM extracts or β2ARKO BM infected with lentiviral-expressed GFP or β2AR and incubated with an AP-1–specific probe. (C) CCR2 expression was quantified in WT BM treated with vehicle, Sal ± SR11302, or SR11302 alone. n = 4–8; one-way ANOVA, ‡P < 0.001. (D) Human monocytes were treated with vehicle, Sal ± SR11302, or SR11302 alone. CCR2 expression was measured using RT-qPCR. n = 6–12; one-way ANOVA, *P < 0.05, †P < 0.01. (E and F) β2ARKO BM was infected with lentiviral-expressed WT β2AR (E) or lenti-β2ARTYY (F) and treated with vehicle, Sal ± SR11302, or SR11302 alone. CCR2 expression was examined by RT-qPCR. n = 4–6; one-way ANOVA, *P < 0.05, †P < 0.01. Data are expressed as mean ± SEM.