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. 2016 Dec 12;113(52):15126–15131. doi: 10.1073/pnas.1611023114

Fig. 7.

Fig. 7.

Restoration of β2AR expression in β2ARKO BM reverses immune dysfunction following MI. (A) RT-qPCR was used to quantify CCR2 expression in reconstituted BM from mice that had GFP, WT β2AR, β2ARTYY, or β2ARGRK− transduced into β2ARKO BM by lentivirus before transplantation. Values are expressed relative to WT BMT. n = 4–8; one-way ANOVA, *P < 0.05, ‡P < 0.001 vs. β2AR+GFP. (B) Representative CD68, tryptase, and MPO staining for the border zone of hearts from WT C57BL/6 mice receiving β2ARKO BM with GFP, WT β2AR, β2ARTYY, or β2ARGRK− transduced before transplantation that underwent MI surgery. Arrowheads indicate positive staining. Insets show higher magnification at 250×. (C–E) Quantification of CD68 (C), tryptase (D), and MPO (E) staining for the border zone of 4-d post-MI hearts from GFP, WT β2AR, β2ARTYY, or β2ARGRK− BMT mice. Arrowheads indicate positive staining. n = 4–8; one-way ANOVA, *P < 0.05, ‡P < 0.001 vs. β2ARKO+β2AR BMT. Data are expressed as mean ± SEM.