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. 2016 Dec 12;113(52):E8425–E8432. doi: 10.1073/pnas.1618548114

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Fig. 5. — Up-regulation of β5i (LMP7) expression in CD4 and CD8 T cells upon alloactivation. Splenocytes of C57BL/6 mice recipients of BALB/c heart at day 7 posttransplantation were analyzed by flow cytometry. (A) Representative histogram showing a significant increase in β5i expression in CD4 effector T cells (CD4CD44^highCD62l^low) compared with naive T cells (CD4CD44^lowCD62l^high). (B) Representative histogram showing a significant increase in β5i expression in CD8 effector T cells (CD8CD44^highCD62l^low) compared with naive T cells (CD8CD44^lowCD62l^high). (C) Mean fluorescence intensity of LMP7 in naive and effector CD4 T cells (n = 3 mice; *P < 0.05). (D) Mean fluorescence intensity of β5i in naive and effector CD8 T cells (n = 3 mice; *P < 0.05).