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(Left) Structural representation of the TEM–BLIP complex (PDB ID code 1JTG). Positions that did not tolerate mutations are presented as spheres (green, C; red, O; blue, N). Residues important for binding are labeled in yellow. Arg243 on TEM forms a salt bridge with Asp49 on BLIP (represented as sticks), which is important for binding. (Right) A table indicating the conserved positions and their kind: 1, interface; 2, buried; 3, surface.
