Table 2.

Sequences and relative k_on values of individual clones resulting from stringent kinetic selection

Clone	Sequence*	TransComp†	konmut/konwt‡
1	I95L A126T	1.3
2	H96R D115G	2.5	3.2
3	K111R I208V T269N	1.4
4	K192N	0.9
5	Y262N	1.0
6	L51F K111N E121D A201T D214N S223P	0.7
7	I56V V80A T141A E171V	1.2
8	E110V T200I M270K	8.6	3.6
9	G91S G116S L162H	0.9	1.1
10	E239K	6.1	5.0
11	E171G E279V	1.4
12	L102R	1.2
13	Q90H K111R A135T V159E T182R	9.7
14	R94H S124G	0.7
15	E63G D115G	2.1	1.2
16	I47T Q88K E110V A227T	3.9	2.6
17	T133A T188A K215R	1.8
18	K111R S223P	2.6
19	Q99R T128I L137S	1.1
20	T188M P219L S266G	1.0
21	L75P E110V M186V I246V	3.8
22	F60L	1.0
23	K111R	3.4	1.9
24	L113R E239K	9.5
25	E110V P226Q	3.8
26	R240A	0.6

^*Interface residues are underlined.

^†TransComp predicted k_on rates at an ionic strength of 0.15 M, taking into account the influence of all occurring mutations, relative to WT.

^‡Stopped-flow measurements as described in Methods, relative to WT (3 × 10⁵ M^-1⋅s⁻¹). The SE for k_onmut/k_onwt is 30%.