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. 2017 Jan 3;7:39760. doi: 10.1038/srep39760

Figure 4. Endogenous expression of pluripotency-related markers by iPSCs.

Figure 4

(A) RT-PCR analysis for detection of the pluripotency markers SOX2, OCT4, NANOG and REX-1. All the patient iPSC clones tested expressed these genes. See full-length gels in Supplementary Figure 2. (B) Immunofluorescence staining of three stem cell proteins (SSEA4, TRA-1-60 and OCT3/4) in patient iPS clones 12 and 32. Both clones 12 and 32, at passages 5 and 4, respectively, expressed the three pluripotency markers used.