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. 2017 Jan 3;7:39864. doi: 10.1038/srep39864

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Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Honey bee samples were collected from 5 different colonies over one year. Protein samples extracted from the heads and abdomens of foragers and nurses were detected by Western blotting using anti-AmAChE1 and anti-AmAChE2 (a). The expression rates of AmAChE1 were normalized by calculating the expression ratio of AmAChE1 vs. AmAChE2 in foragers (b) and nurses (c).