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Changes in the AmAChE1 expression of overwintering honey bees following placement in a greenhouse (a) and field (b). Overwintering beehives were placed in a greenhouse for 72 days to resume brood rearing in winter (a). The beehives were moved back to the field to suppress brood rearing in winter (b). Two AChEs were detected by Western blotting (Fig. S2), and the expression rates of AmAChE1 were normalized by calculating the expression ratio of AmAChE1 vs. AmAChE2.
