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. 2017 Jan 3;7:39864. doi: 10.1038/srep39864

Figure 3. Induction of AmAChE1 expression in overwintering honey bee by supplementing with an artificial diet.

Figure 3

Protein samples were prepared from nine overwintering beehives every month from Dec. 24, 2013 to Mar. 31, 2014. On Dec. 24, 2013, an artificial diet was initially supplied to Colonies 1, 2 and 3 (a). Colonies 1 to 6 and all of the colonies were supplied with the artificial diet on Jan. 22, 2014 (b) and Mar. 01, 2014 (c), respectively. A month later (on Mar. 31, 2014), protein samples, prepared every month, were separated in a native-PAGE gel, and two AChEs were detected by Western blotting. Asterisks indicate the period of artificial diet supplementation.