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. 2017 Jan 3;7:39794. doi: 10.1038/srep39794

Figure 3. Adipocytes with UCP1 expression history become FASNhi and MACDhi adipocytes.

Figure 3

(A,B) Representative images of paraffin sections of BAT (A) and iWAT (B) double-stained for UCP1 and tdTomato, and quantification of UCP1+tdTomato+/tdTomato+ adipocytes. (C) Representative images of paraffin sections of iWAT double-stained for FASN and tdTomato, and quantification of populations of tdTomato+ adipocytes distinguished by cellular morphology and FASN staining (n = 3 mice (>100 tdTomato+ adipocytes per animal). Arrows indicate tdTomato+FASN+ adipocytes. (D) Representative images of paraffin sections of iWAT double-stained for MCAD and tdTomato, and quantification of population of tdTomato+ adipocytes distinguished by cellular morphology and MCAD staining (n = 3 mice (>100 tdTomato+ adipocytes per animal). Arrows indicate tdTomato+MCAD+adipocytes. (E) Representative images of paraffin sections of iWAT triple-stained for FASN, UCP1 and tdTomato. Arrows indicate tdTomato+UCP1+ adipocytes whereas arrowheads indicate FASN+tdTomato+ adipocytes. Size bars = 40 μm.