H&E histology of the targeted nerve and adjacent muscle. Compared to normal muscle (A), ablated muscle showed swelling of muscle fibres, sarcoplasmic hypereosinophilia and fragmentation, and nuclear shrinkage (B) with macrophages present at the boundary of the lesion in subacute cases (arrow, C). 50 µm scale bar. Compared to the control nerve (D), the ablated nerve exhibited at least one, and often a combination, of the following changes: hyalinization of epineurial collagen (asterisk, E), nuclear shrinkage (arrow, E, F), hyperemia of endoneurial vessels (long arrow, F), and loss of axons associated with dilation of the myelin sheath (arrowhead, F). 20 µm scale bar.