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. 2017 Jan 3;7:39883. doi: 10.1038/srep39883

Figure 3. Validation of MeDIP-seq data by bisulfite sequencing PCR.

Figure 3

(A) A low methylated region across the TC, LD, and WZS pigs in the promoter of the ENSSSCG00000000890 gene on Chromosome 5 from 91,290,663 to 91,291,043. (B) A differentially methylated region on Chromosome X from 114,141,608 to 114,142,169. (C) A differentially methylated region across the three pig breeds on Chromosome 15 from 145,240,683 to 145,241,082. (D) A differentially methylated region across the three pig breeds on Chromosome 10 from 54,905,527 to 54,905,886. The visualization of DNA methylation signal of MeDIP-seq along the chromosome in each pig breed were performed by Integrative Genomics Viewer (top panels). The bottom panels show the results of bisulfite sequencing PCR. Each box corresponds to one CpG position in the genomic sequence. The colored bars summarize the methylation states of all sequences at that position, blue indicates unmethylated and yellow indicates methylated.