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. 2017 Jan 3;7:39901. doi: 10.1038/srep39901

Figure 1. Silencing of TAK1 using siRNA, or treatment with TAK1 inhibitor (5-Z-7-Oxozeaenol), enhances HBV replication and gene expression in cell culture.

Figure 1

(A) HepG2.2.15 cells were transfected with validated TAK1 siRNA or control siRNA, as indicated, and cultured for 4 days. Cell lysates and culture supernatants were harvested on day 4 after transfection, levels of HBV DNA replicative intermediates were determined by Southern blot analysis (top panel). TAK1, intracellular HBcAg expression and core particles were analyzed by Western blotting with β-actin as loading control (middle panel). HBsAg and HBeAg were detected in the culture supernatants by CMIA assay (bottom panel). (B) HepG2.2.15 cells were treated with the specific TAK1 inhibitor, 5-Z-7-Oxozeaenol (5Z-7-Ox), at the indicated concentrations for 4 days. Cell lysates and culture supernatants were harvested on day 4. Levels of HBV replicative intermediates (top panel), TAK1, intracellular HBcAg expression, core particles (middle panel), and levels of HBsAg and HBeAg (bottom panel) were determined as described above. RC, relaxed circular; DL, double stranded linear; SS, single stranded.