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Purification of the ATRX fragment (ATRXepi) from transformed E. coli. The soluble fraction (5 μL) from the transformed E. coli after PMab-1-Sepharose capture (flowthrough), and the fifth of five washes in 20 mL TBS (wash), and 10 peptide-elution fractions (lanes 1–10) from the column chromatography were subjected to 5%–20% SDS-PAGE under reducing conditions and stained with CBB. TBS, Tris-buffered saline; CBB, Coomassie Brilliant Blue.
