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. 2016 Dec 1;35(6):293–299. doi: 10.1089/mab.2016.0039

FIG. 4.

FIG. 4.

Purification of EGFR from LN229 cells. PMab-1-Sepharose captured the proteins in the detergent-solubilized cell lysates from transfected LN229 and was washed with 20 mL PBS. (A) Ten peptide-elution fractions (10 μL) (lanes 1–10) from the column chromatography were subjected to 5%–20% SDS-PAGE under reducing conditions and stained with Oriole fluorescent protein stain. (B) Ten peptide-elution fractions (5 μL) (lanes 1–10) from the column chromatography were subjected to 5%–20% SDS-PAGE under reducing conditions and transferred to a membrane. The membrane was immunoblotted with 1 μg/mL of PMab-2 (anti-RAP tag) for 30 minutes and incubated with peroxidase-conjugated secondary antibody specific for mouse IgG. PBS, phosphate-buffered saline; SS, signal sequence.