FIG. 2.

PMab-38 reacted with PDPN of melanoma cells, cancer-associated fibroblasts, and lymphatic endothelial cells. Sections of melanomas (A, case 7; B, case 9; C, case 2) were incubated with 10 μg/mL of PMab-38 overnight at 4°C followed by treatment with Envision+ kit for 30 minutes (Dako). As a control, blocking buffer was used. Color was developed using 3,3-diaminobenzidine tetrahydrochloride (Dako) for 2 minutes, after which the sections were counterstained with hematoxylin (Wako). H&E staining was also performed. Scale bar: 100 μm. (A) staining of melanoma cells by PMab-38, (B) staining of cancer-associated fibroblasts by PMab-38, and (C) staining of lymphatic endothelial cells by PMab-38.