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. 2017 Jan 3;7:39501. doi: 10.1038/srep39501

Figure 4. Plumericin, PHA-408, and NAC prevent TNFα-induced nuclear translocation of NF-κB and ROS generation in HUVECs.

Figure 4

(a) Quantification of nuclear translocation of NF-κB in HUVECs grown in the presence of TNFα (10 ng/ml) ± inhibitors in comparison to control HUVECs ± inhibitors at 20 min, by integrated mean intensity of p65 in nucleus using CellProfiler software. (b–f) Determination of ROS production in HUVECs grown in the presence of TNFα (10 ng/ml) ± inhibitors in comparison to control HUVECs ± inhibitors for indicated time points, by using fluorophore H2-DCF (10 μM). Values are presented as mean ± SD of technical triplicates (*p < 0.05, **p < 0.01, ***p < 0.001). The results shown are representative of three independent experiments. C: control, PL: plumericin, PHA: PHA-408, NAC: N-acetyl cysteine, a.u.: arbitrary units.